Characterization and Development of EST-Derived SSR Markers in Sinowilsonia henryi (Hamamelidaceae)
نویسندگان
چکیده
منابع مشابه
Characterization and development of EST-derived SSR markers in Sinowilsonia henryi (Hamamelidaceae)1
Premise of the study Polymorphic microsatellite markers were developed to reveal the genetic diversity of extant populations and the mating system of Sinowilsonia henryi (Hamamelidaceae). Methods and Results In this study, nuclear simple sequence repeat (SSR) markers were developed using the Illumina high-throughput sequencing technique (RNA-Seq). The de novo-assembled transcriptome generated...
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In this study, a cDNA library was constructed from the total RNA of sweet potato leaves. A total of 789 copies of the cDNA were cloned in Escherichia coli by employing the pGEM-T Easy vector. Sequencing was carried out by Solgent Co. (Korea). As many as 579 expressed sequence tag-simple sequence repeat (EST-SSR) markers were designed (73.38%) from the known cDNA nucleotide base sequences. The l...
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ژورنال
عنوان ژورنال: Applications in Plant Sciences
سال: 2017
ISSN: 2168-0450
DOI: 10.3732/apps.1700080